The same as the standard product, the control product is also a "frequent guest" in the laboratory, some people say that the standard product and the control product are like twin brothers, this is actually true, although there is an inevitable difference between the two, but the relationship is inseparable, since it is a frequent visitor to the laboratory, its use is mainly used for biochemical research, content determination, etc., according to incomplete statistics, The varieties of reference products have reached hundreds or even thousands of kinds, so many reference products, how should we correctly use it? The following small series to introduce you to the correct use of reference products.

Proper use of reference:

For the use of reference products, we are generally divided into four steps, such as opening, opening, etc., the specific correct use of the method please see below:

1. Correct opening (when the control product is opened)

Many of the control products are stored in glass ampoules, which must be strictly prevented from dropping glass chips into the bottle when opened. Specific operations can be as follows: slightly knock the ampoule, as far as possible to make the contents gathered at the bottom, after a few rounds of grinding wheel cutting, flat or slightly downward Angle the ampoule, wear protective gloves, the thumb of both hands against the neck force, pay attention to the thumb and index finger do not pinch too tight, so as not to crush the head. After opening the ampoule, the ampoule slightly downward tilt, slight vibration knock, because the glass debris is heavier, if mixed with the contents, this method can shake it out.

2. After the control product is opened

It should be strictly sealed, or transferred to the Xilin bottle cooled to room temperature in the dryer after drying at more than 100℃, sealed, placed in the dryer, some varieties should be refrigerated in the refrigerator according to the instructions; For reference products that are easy to absorb moisture, such as omeprazole, if used for content determination, it should be used immediately after opening, and the remaining part can be used for identification in the future, but it is recommended not to be used for content determination; For varieties with weak humidification and good stability, such as chloramphenicol, if properly preserved after opening, it can also be used for content determination, and the use time depends on the storage conditions, and the period of inspection should be done if necessary.

3. Expired reference products

For expired reference products, if they continue to be stored, they need to be placed separately to avoid confusion; If necessary (such as out of stock), can also be used in emergency, can be used for identification; If used for quantitative purposes, traceability verification data must be guaranteed.

4. Storage requirements of reference products

A. The storage conditions of the reference product solution are generally stored in the refrigerator at 2 ~ 8 ° C, and the interval time limit can be determined according to the specific characteristics of the reference product solution; According to the drug quality standard, the reference solution is prepared, the content of the reference solution is calculated, and the normal product test is carried out with the reference solution according to the quality standard.

b, the control product solution is placed to the first period (usually 3 days, 7 days, 1 month, etc., according to the stability of the control product), according to the drug quality standards, prepare a new control product solution (according to the drug quality standards for testing) to calculate the content.

c, at the same time, the initial reference solution (as a product) is tested according to drug quality standards, and the content is calculated; The relative deviation of the obtained content is calculated, if it is not greater than 1.0%(some provisions are not greater than 2.0%, personally feel that the deviation is a little large), it means that the reference solution or the standard solution is stable and can be used for testing within the first period; Repeat to determine the specific storage time of the control product.

The above content is the introduction of the correct use of the reference products, in view of the variety of reference products, for its preservation method we are also certain requirements, generally speaking, the reference products should be placed in a dry and cool place, if improperly preserved, will directly affect its experimental accuracy, but for different reference products, the preservation method is also different. It is recommended to use the control product after opening the bottle as soon as possible to avoid the decomposition, pollution or moisture absorption of the control product.